Reporter Cell Lines

Do you have trouble tracking the engrafted cells in vivo? Do you want to monitor gene expression or protein trafficking dynamically? If the answer is yes, the reporter cell line will be an ideal tool for you.

Benefited from the discovery of reporter genes/proteins (e.g., green fluorescent protein (GFP), luciferase), cell lines are engineered to integrate reporter expression cassettes into its genomes. With the ‘visible’ tag, these reporter cell lines are valuable tools for various scientific studies，such as analysis of gene expression and regulation, cell labeling and in vivo imaging, tumor evaluation, and drug screening.

With robust virus production technology as well as years of experience, scientists at Alstem have established a growing library of reporter cell lines to meet your experimental demand. Our current collections include but not limit to luciferase/GFP, luciferase/tdTomato, nanoluciferase/tdTomato dual reporters cell lines. The type of donor cell covers HEK293, HeLa, Jurkat, A375, HCT116, MC38, etc.

Description：Luciferase catalyzes the substrate luciferin in the presence of Mg2+ and ATP, resulting in a flash of light that decays rapidly. This light can be detected by a luminometer. Luciferase from the firefly has become one of the more widely used reporter proteins for both in vitro and in vivo studies, such as, gene transcription activity assay, the noninvasive imaging of reporter gene expression, imaging cerebral strokes, and tracking genetically engineered T cells. Luciferase/GFP dual-reporter HCT116 (HCT116-Luc-GFP) cell line is derived from HCT116 cells by transduction of ALSTEM’s pLenti-CAG-Luciferase-PGK-GFP-T2A-PURO Lentiviral Reporter (cat# LV311G) lentivirus, which contains Firefly luciferase encoding gene driven by CAG promoter, followed by GFP and puromycin resistance gene by PGK promoter. This cell line stably expresses luciferase at high level. It also expresses a green fluorescent protein (GFP) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.

Cell Line: HCT116

Reporters: Luciferase, GFP

Selection Marker: Puromycin

Promoter/Transgene: CAG-Luc-PGK-GFP-T2A-puro

Cell Type: Single Clone

Figure 1. Panel A: GFP Fluorescence. Dual-labeled HCT116 cell line expressing GFP at 90% cell confluence. Panel B: Dose-response luciferase activity analyses in HCT116 cell line after Staurospine treatment. Shown is luciferase activity change of HCT116 cells after incubation with increasing concentrations (μM) of Staurospine for 24h. Data are expressed as mean /- SD from duplicates of three independent experiments. The luciferase activity was tested 6 hrs after seeding. Data are expressed as mean /- SD from duplicates of three independent experiments.
Figure 2. The lentiviral reporter vector contains luciferase gene driven by CAG promoter, GFP and puromycin resistance gene by PGK promoter.

Specifications: