锐赛生物，只为您更专注
我们不止关注您所专注的实验

28 July 2014
Springer：Protective effects of adiponectin on uncoupling of glomerular
International Urology and
Nephrology
ISSN 0301-1623
Int Urol Nephrol
DOI 10.1007/s11255-014-0807-x



Abstract
Purpose To determine whether adiponectin could reduce
microalbuminuria and provide renal protective effects by
improving endothelial dysfunction and uncoupling of the
glomerular vascular endothelial growth factor (VEGF)–
nitric oxide (NO) axis in streptozotocin-induced type 2
diabetic rats.
Methods Wistar rats were randomly divided into normal
control group, diabetic nephropathy (DN) group induced by
high-fat feeding and streptozotocin, diabetic rats injected
with adenovirus-expressed adiponectin (AD-AdipoQ), and
diabetic rats injected with AD-IRES-EGFP as control.
Blood and urine samples were collected. Endotheliumdependent
vasodilatation (EDV) of the aorta was measured.
Renal tissues were collected for CD34 immunohistochemistry.
Glomerular NO and VEGF levels were measured by
the Griess reaction and Western blot testing, respectively.
Results Injections of AD-AdipoQ significantly increased
serum adiponectin levels and reduced the urinary albuminto-
creatinine ratio in diabetic rats (P\0.05). The levels of
plasma glucose, serum insulin, high-sensitivity C-reactive
protein, and malondialdehyde were significantly reduced in
diabetic rats after injections of AD-AdipoQ (P\0.05).
Severe EDV impairment was observed in the DN group,
which was improved by AD-AdipoQ. CD34 expression in
the glomeruli was also higher in diabetic rats, indicating


Adenovirus expressing
adiponectin (AD-AdipoQ-IRES-EGFP, AD-AdipoQ) and
control AD-IRES-EGFP (AD-IRES) were constructed by
Shanghai R&S Biotechnology Co., Ltd (Shanghai, China).